A new virus isolated from cultured grass carp Ctenopharyngodon idella

W Ahne, Y Jiang, I Thomsen
1987 Diseases of Aquatic Organisms  
A new virus isolated from cultured grass carp Ctenopharyngodon idella ABSTRACT: A virus isolated from normal-appearing grass carp (Ctenopharyngodon idella Valenciennes, 1844) multiplied in fish cells (CAR and CLC cells) at 15 to 25 ' C producing syncytia followed by lysis. The agent was inactivated by treatment with acid (pH 3), heat (56 "C), and chloroform (50 %). Human type 0 erythrocytes were not hemagglutinated (pH 7.2, 20 "C). Treatment with 5-iodo-2deoxyuridine did not affect viral
more » ... tion, indicating an RNA genome. Electron microscopy of infectious tissue culture fluid revealed rod-shaped particles measuring 170 to 220 X 50 to 55 nm.
doi:10.3354/dao003181 fatcat:rwb3am7vjnervmg5kctg2tp2vm