Rapid Selection of High-Affinity Binders Using Ribosome Display [chapter]

Birgit Dreier, Andreas Plückthun
2011 Msphere  
Ribosome display has proven to be a powerful in vitro selection and evolution method for generating high-affi nity binders from libraries of folded proteins. It has been successfully applied to single-chain Fv fragments of antibodies and alternative scaffolds, such as D esigned A nkyrin R epeat P roteins (DARPins). High-affi nity binders with new target specifi city can be obtained from highly diverse DARPin libraries in only a few selection rounds. In this protocol, the selection from the
more » ... ry and the process of affi nity maturation and off-rate selection are explained in detail.
doi:10.1007/978-1-61779-379-0_15 pmid:22094811 fatcat:ygjo747cgjfjjca44pzkvgwq6a