Overexpression of a basic helix–loop–helix gene Antagonist of PGL1 (APG) decreases grain length of rice

Dany Heang, Hidenori Sassa
2012 Plant Biotechnology  
Previously we reported that three basic helix-loop-helix (bHLH) protein dimers are involved in regulating grain length of rice. Two atypical bHLH proteins named P (PGL1) and PGL2 positively regulate grain length in transgenic rice when they are overexpressed in lemma/palea. eir interaction partner A PGL (APG), a typical bHLH, is assumed to have opposite function. Suppression of APG expression level by RNAi increased grain length signi cantly. Here, we report further evidence that APG is a
more » ... that APG is a negative regulator of grain length in rice. Overexpression of APG resulted in short grain. Inner epidermal cell layer of lemma from APG overexpression lines showed reduced cell length. Taken together, the results illustrated the biological role of a typical bHLH APG as a negative regulator of rice grain length that controls cell elongation. Abbreviations: APG, Antagonist of PGL1; bHLH, basic helix-loop-helix; PGL1 and 2, Positive regulator of grain length 1 and 2. is article can be found at
doi:10.5511/plantbiotechnology.12.0117a fatcat:xv5rgqujwveebihqlt4r26cdse