In-ovulo embryo culture in interspecific crosses of Gossypium

R. Pushpam, R. Ravikesavan, T.S. Raveendran
2017 Electronic Journal of Plant Breeding  
The immature hybrid embryos of five back crosses viz., (MCU 5 x G. armourianum) MCU 5, (MCU 5 x G. raimondii) MCU 5, (MCU 12 x G. raimondii) MCU 12, (TCH 1569 x G. armourianum) TCH 1569,(TCH 1609 x G. raimondii) TCH 1609 were excised 8 days after pollination and were cultured in various concentrations of auxin and cytokinin. To prevent boll shedding in all the crosses, growth hormones NAA 100 mg/l and GA 3 50 mg/l was applied at the base of the pedicel for 8 days and then the ovules were
more » ... ovules were excised and cultured on media to form calli. Out of different media combinations tried, maximum callus induction was observed in the medium containing IAA 2.0 mg/l, Kn 1.0 mg/l, CH 250 mg/l, AS 40 mg/l in all the crosses. Maximum response of callus induction was observed in the cross (MCU 5 x G. raimondii) MCU5.
doi:10.5958/0975-928x.2017.00183.1 fatcat:2wcukbkrtnaano24kzbdbmomgq