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The presence of covalently linked ribonucleotides in the closed circular deoxyribonucleic acid from higher plants
Journal of Biological Chemistry
Single-stranded scissions are induced in the covalently closed circular chloroplast (ct-) DNAs from peas, spinach, and lettuce plants by treatment with alkali or by incubation with a mixture of ribonucleases A and T1. These scissions are due to the presence of covalently linked ribonucleotides in these closed circular DNAs. By comparing the scission rates of these ctDNAs to the scission rate of RNA, it has been estimated that pea and spinach ctDNAs contain a maximum of 18 +/- 2pmid:1158895 fatcat:sjlievnambfsto2depxeorfkce