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The species of Asparagus are very important as they are used for ornamental, vegetable and medicinal purposes since ancient time. In the present study, random amplified polymorphic DNA (RAPD) markers were used to evaluate genetic diversity among nine species of Asparagus and six cultivars of Asparagus officinalis L. RAPD analysis using seven random oligonucleotide primers yielded a total amplification of 245 bands, among which 220 (89.80%) were polymorphic with an average of 31.4 bands perdoi:10.5897/ijbc2014.0708 fatcat:m5twzjh2zrdexehhdxkdm43qci