Pegylated-Interferon-alpha treatment modifying T cell cytokine profile in tumor microenvironment of patients with cervical intraepithelial neoplasia

2021 European Journal of Gynaecological Oncology  
Objective: The goal of the investigation was to compare the profiles the cytokines IL-2, IL-12, IFN-γ, TNF-α , TGF-β, IL-4, IL-6 in the endocervical and serum secretions of patients with cervical intraepithelial neoplasia (CIN) who had good or poor response to immunotherapy with pegylated (PEG)-Interferon-alpha. Methods: The study was performed with 16 patients who had been diagnosed with CIN II and III. Each patient was submitted to six injections of PEG-Interferon-α -2b subcutaneously. A ter
more » ... cutaneously. A ter each injection, endocervical secretion and serum samples were collected for cytokine levels by ELISA. Results: Of the 16 women, 43.75% (n = 7) showed good clinical response and lesion regression a ter treatment as seen by histopathological analysis. When we analyzed the pre-therapy, locally we observed a higher concentration of IL-4 in patients who did not respond to treatment (P = 0.0229). A ter treatment, there was a significant reduction in IL-4 in unresponsive patients (P = 0.0304). Patients responsive to IFN-α had a reduction in the concentrations the TNF-α a ter treatment (P = 0.0313). Conclusion: Immunotherapy with PEG-Interferon-α can reduce the damage caused by HPV by decreasing local in lammation by reducing the cytokines IL-4 and TNF-alpha during treatment in endocervical secretion. It seems that the regression is related to the immunological profile, mainly local, before treatment, as in the case of IL-4 and TNF-alpha, promoting a Th2 profile in those patients with therapeutic failure.
doi:10.31083/j.ejgo.2021.01.2265 fatcat:kkvf5a6yabc3vdykdbf73olbqm