HIF-1α plays a key role in the response of HNSCC cancer stem cells to photon and carbon ion exposures

A.-S. Wozny, A. Lauret, Y. Saintigny, P. Battiston-Montagne, M. Beuve, G. Alphonse, C. Rodriguez-Lafrasse
2016 Radiotherapy and Oncology  
ICTR-PHE 2016 S115 technology allows highly multiparameter (up to 34 probes) cytometric or image based quantification of biomarkers on single cells. MC employs inductively coupled plasma mass spectrometric detection in place of the fluorescence optics used in traditional flow and imaging cytometry and circumvents the problems of spectral overlap and compensation required in fluorescence flow cytometry. The key feature of MC that we have exploited is that different mass isotopes of the same
more » ... es of the same heavy metal can be independently quantified and thus used as distinct probes to allow serial measurements over time with the same chemical probe. This has allowed a series of probes that have identical pharmacokinetic profiles to be produced and used to monitor the time dependence of tumor hypoxia in both in vitro and in vivo model systems.
doi:10.1016/s0167-8140(16)30237-7 fatcat:dqnsqpu5vrh6di3ytghhz4f2ta