Upregulated IL-23 and IL-17 in Behçet Patients with Active Uveitis

Wei Chi, Xuefei Zhu, Peizeng Yang, Xiaoli Liu, Xiaomin Lin, Hongyan Zhou, Xiangkun Huang, Aize Kijlstra
2008 Investigative Ophthalmology and Visual Science  
PURPOSE. Behçet disease (BD) is a systemic inflammatory disease presumably caused by an autoimmune response. The interleukin (IL)-23/IL-17 pathway has been demonstrated to be involved in the development and maintenance of certain inflammatory diseases. This study was designed to investigate the role of IL-23 and IL-17 in BD. METHODS. IL-23p19 mRNA in peripheral blood mononuclear cells (PBMCs) was examined using RT-PCR. The levels of IL-23, IL-17, and IFN-␥ in sera or PBMCs were detected by
more » ... . Flow cytometry was used to evaluate the frequencies of IL-17producing and IFN-␥-producing T cells and the expression of CD45RO. RESULTS. Results showed that the expression of IL-23p19 mRNA, IL-23, IL-17, and IFN-␥ was markedly elevated in BD patients with active uveitis. The frequencies of IL-17-producing and IFN-␥-producing T cells from PBMCs were significantly upregulated in BD patients with active uveitis. The increased IL-17 (3.10% Ϯ 0.53%) in BD patients with active uveitis was primarily produced by CD45RO ϩ memory T cells. Recombinant (r) IL-23 could upregulate IL-17 production by polyclonally stimulated PBMCs, whereas interferon (IFN)-␥ downregulated IL-17 production. CONCLUSIONS. These findings reveal that the levels of IL-23, IL-17, and IFN-␥ are elevated in BD patients with active uveitis, and they suggest that the IL-23/IL-17 pathway together with IFN-␥ is associated with the active intraocular inflammation in
doi:10.1167/iovs.07-1390 pmid:18579762 fatcat:4ga3wiwxzzdaxhz6cy5kgfzcg4