PHYSICAL AND CHEMICAL CHARACTERISTICS OF CHLOROPLAST FRAGMENTS. APPLIED RESEARCH CONCERNING ARTIFICIAL PHOTOSYNTHESIS [report]

JEAN A. GROSS, MILTON J. BECKER, ALAN M. SHEFNER
1962 unpublished
Chloroplasts isolated from spinach leaves were physically fragmented and then fractionated by differential centrifugation. A high-activity fraction, designated CF 2 0 _ 5 0 , was obtained and retained its high activity regardless of environmental changes. It had a low protein content and a relatively high chlorophyll-to-protein ratio. Plastoquinone was present in high concentration. Extraction of plastoquinone from CF 2 0 . 5 0 inhibited Hill activity. Unlike whole chloroplasts, readdition of
more » ... astoquinone failed to induce even partial recovery.
doi:10.21236/ad0296951 fatcat:5fvj3m4yfvdqbcuh2vl5l4txsu