Sero-occurrence of anti-Toxoplasma gondii antibodies and vertical transmission in slaughtered beef cows (Bos indicus)

Joao Luis Garcia, Francisco Augusto C Marques, Odilon Vidotto, Italmar Teodorico Navarro, Guilherme Felippelli Martins, Dauton Luiz Zulpo, Ivo Alexandre L da Cunha, Alessandra Taroda, Sergio Tosi Cardim, Maria Paula de C Ewald
2012 Semina: Ciências Agrárias  
Toxoplasma gondii is a protozoan parasite recognized as an important public health problem. The objective of the present study was to evaluate the occurrence of anti-T. gondii antibodies in pregnant and non pregnant zebus' breed beef cows (Bos indicus), their fetuses, killed at an abattoir in northern of Paraná state. In the present study 169 cows were evaluated, 92 pregnant (in different stages of gestation) and 77 non pregnant. Sero-occurrence of anti-T. gondii antibodies was performed by
more » ... as performed by Indirect fluorescent antibody test (IFAT) considering positive animals with titers ≥ 50 for cows and ≥ 25 for fetuses. Blood (with EDTA) from pregnant cows and blood and tissue samples (brain, lung, heart, and liver) from their fetuses were collected and used for mouse bioassay. Antibodies against T. gondii were observed in 26.0% of cows and 2.5% of fetuses. There was no statistical difference when prevalence of toxoplasmosis was compared between pregnant (23.9%), and non-pregnant (28.6%) animals, and age of gestation (p > 0.59). However, the occurrence of anti-T.gondii antibodies increased with age of animals (p=0.004). Mouse bioassay showed three fetuses positives (3.2%), however, none T. gondii strain was isolated. The present study showed that transplacental transmission of T. gondii naturally occurs in zebu beef cows from Brazil, however, in low rate (5.4%). The anti-T. gondii antibodies occurrence increase with the age of animals, which could be related to the fact that main transmission in cattle T. gondii is horizontal. Semina: Ciências Agrárias, Londrina, v. 33, n. 3, p. 1095-1102, maio/jun. 2012 Garcia, J. L. et al. em camundongos. Anticorpos contra T. gondii foram observados em 26,0% das vacas e em 2,5% dos fetos Não houve diferença quando a soropositividade de anticorpos foi comparado entre vacas gestantes (23,9%), e não gestantes (28,6%), bem como, a idade da gestação (p > 0,59). No entanto, a ocorrência de anticorpos aumentou com a idade dos animais (p = 0,004). O bioensaio mostrou três fetos positivos (3,2%), porém, nenhuma cepa foi isolada. O presente estudo mostrou que a transmissão transplacentária de T. gondii ocorre naturalmente em vacas de corte zebuínas do Brasil, no entanto, esta ocorrência foi baixa (5,4%). A maior ocorrência de anticorpos associada com a idade dos animais poderia estar relacionada a transmissão horizontal do T. gondii nestes animais. Palavras-chave: Toxoplasmose, zebu, transmissão vertical
doi:10.5433/1679-0359.2012v33n3p1095 fatcat:gnrakxg3vjh4jent36236o25pm