Co-loading antioxidant N-acetylcysteine attenuates cytotoxicity of iron oxide nanoparticles in hypoxia/reoxygenation cardiomyocytes

Yunli Shen, Shiyu Gong, Jiming Li, Yunkai Wang, Xumin Zhang, Hao Zheng, Qi Zhang, Jieyun You, Zheyong Huang, Yihan Chen
2019 International Journal of Nanomedicine  
These authors contributed equally to this work Purpose: Myocardial delivery of magnetic iron oxide nanoparticles (MNPs) might produce iron overload-induced myocardial injury, and the oxidative stress was regarded as the main mechanism. Therefore, we speculated antioxidant modification might be a reasonable strategy to mitigate the toxicity of MNPs. Methods and results: Antioxidant N-acetylcysteine (NAC) was loaded into magnetic mesoporous silica coated Fe 3 O 4 nanoparticles. Neonatal rat
more » ... Neonatal rat hypoxia/reoxygenation (H/R) cardiomyocytes were incubated with nanoparticles for 24 hrs. NAC can effectively mitigate iron-induced oxidative injury of cardiomyocytes, evidenced by reduced production of MDA, 8-iso-PGF2α, and 8-OHDG and maintained concentrations of SOD, CAT, GSH-Px, and GSH in ELISA and biochemical tests; downregulated expression of CHOP, GRP78, p62, and LC3-II proteins in Western Blot, and less cardiomyocytes apoptosis in flow cytometric analysis. Conclusions: NAC modifying could suppress the toxic effects of Fe 3 O 4 nanoparticles in H/ R cardiomyocytes model in vitro, indicating a promising strategy to improve the safety of iron oxide nanoparticles.
doi:10.2147/ijn.s209820 fatcat:ewbaggpbhvfzfmd6e3x23cqriy