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This study was conducted to detect the prevalence of OXA-181 gene producing Escherichia coli and Klebsiella spp. in a tertiary care hospital of Bangladesh. Total 166 Escherichia coli and Klebsiella spp. were isolated from urine, wound swab, pus, blood and sputum samples in Dhaka Medical College Hospital. Antibiotic susceptibility test was performed by disk-diffusion technique. Carbapenemase producers were detected phenotypically by Modified Hodge test. Carbapenemase genes (blaNDM-1, blaVIM,doi:10.18801/jmsmr.030118.15 fatcat:gwx2v7slu5ddtl7kkapioqi6oi