OXA-181- an emerging threat in Bangladesh

S. Satter, H. Mahbub, S. M. Shamsuzzaman
2018 Journal of Molecular Studies and Medicine Research  
This study was conducted to detect the prevalence of OXA-181 gene producing Escherichia coli and Klebsiella spp. in a tertiary care hospital of Bangladesh. Total 166 Escherichia coli and Klebsiella spp. were isolated from urine, wound swab, pus, blood and sputum samples in Dhaka Medical College Hospital. Antibiotic susceptibility test was performed by disk-diffusion technique. Carbapenemase producers were detected phenotypically by Modified Hodge test. Carbapenemase genes (blaNDM-1, blaVIM,
more » ... aNDM-1, blaVIM, blaIMP, blaKPC, blaOXA-48/blaOXA-181) among imipenem resistant strains among the isolated Escherichia coli and Klebsiella spp. were detected by PCR. Sequencing was done to differentiate blaOXA-181 from blaOXA-48. Thirty-seven (22.29%) imipenem resistant isolates were detected during disk-diffusion technique, among them 43.24% carbapenemase producers were detected by MHT and 62.16% by PCR. NDM-1 (43.24%) was the dominant genotype followed by KPC (21.62%) and OXA-181 (18.92%). The results of this study showed presence of high proportion of carbapenemase enzyme producing Escherichia coli and Klebsiella spp. in Bangladesh. BlaOXA-181 gene is emerging in Bangladesh.
doi:10.18801/jmsmr.030118.15 fatcat:gwx2v7slu5ddtl7kkapioqi6oi