Quantifying drug tissue biodistribution by integrating high content screening with deep-learning analysis

Zhuyin Li, Youping Xiao, Jia Peng, Darren Locke, Derek Holmes, Lei Li, Shannon Hamilton, Erica Cook, Larnie Myer, Dana Vanderwall, Normand Cloutier, Akbar M. Siddiqui (+4 others)
2020 Scientific Reports  
Quantitatively determining in vivo achievable drug concentrations in targeted organs of animal models and subsequent target engagement confirmation is a challenge to drug discovery and translation due to lack of bioassay technologies that can discriminate drug binding with different mechanisms. We have developed a multiplexed and high-throughput method to quantify drug distribution in tissues by integrating high content screening (HCS) with U-Net based deep learning (DL) image analysis models.
more » ... his technology combination allowed direct visualization and quantification of biologics drug binding in targeted tissues with cellular resolution, thus enabling biologists to objectively determine drug binding kinetics.
doi:10.1038/s41598-020-71347-6 pmid:32873881 fatcat:yagj7jrsrrbizj3r7mshziq4ae