Validation of ELISA Kits for Pesticide Residue Analysis in Vegetables and Fruits
ELISAキットによる野菜・果実中残留農薬分析の妥当性評価の試み

Tomomi YAMASAKI, Tomomi INOUE, Yuki HIRAKAWA, Shiro MIYAKE, Eiji UENO, Isao SAITO
2015 Shokuhin Eiseigaku Zasshi  
Five kinds of commercially available ELISA kits acetamiprid, azoxystrobin, chlorothalonil, fenitrothion and imidacloprid were validated for determination of pesticide residues in vegetables and fruits. The reaction characteristics were also examined to evaluate their influence on the determinations. The trueness value was 91-162 , the repeatability was 2.1-16.2 , and the reproducibility was 4.0-20.3 . The desired values were achieved for 18 among 30 combinations 60 of the ELISA kits and the
more » ... cultural products examined. A standard curve was necessary for each of the ELISA examinations. The matrix of the agricultural products and pipetting skill of the lab technician both influenced the measurment results.
doi:10.3358/shokueishi.56.240 pmid:26699271 fatcat:nazigkcsg5egpbcsvydgrl7z3m