Protective effect of sodium butyrate on the neonatal mouse model of necrotizing enterocolitis

Qiu-ping LI, Jia-lin YU, Kun HU, Yu HE, Sa XIAO, Ting HOU, Qing AI
2018 Medical Journal of Chinese People's Liberation Army  
Objective To investigate the protective effect of sodium butyrate on the neonatal mouse model of necrotizing enterocolitis and analyze its possible mechanism. Methods Sixty c57BL/6 neonatal mice were randomly divided into two groups (n=30): PBS group and butyric acid group. At the third day after birth, mice in both groups were respectively given PBS and sodium butyrate solution by gavage once a day for 7 days, and neonatal necrotizing enterocolitis (NEC) model was established by hypoxia, cold
more » ... timulation and artificial feeding. The newborn mice were sacrificed overnight after modeling. HE staining and double-blind pathological score were performed to observe the pathological changes of ileocecal intestinal tissue. The mRNA expressions of IL-6, IL-10, TGF-β1 and TNF-a were tested by quantitative real-time PCR. The levels of IL-10 and TGF-β1 in intestine tissues were evaluated using ELISA. Flow cytometry was used to analyze the ratio of regulatory T cells (Treg) on CD4+ T cells in both groups. Results When mice were sacrificed overnight after NEC modeling, the body weight was significantly higher in butyric acid group (4.50±0.42g) than in PBS group (4.16±0.60g, P<0.05); No significant difference (P>0.05) existed in survival rate of butyric acid group (76.34%) and PBS group (67.95%). The pathological damage score of intestinal tissue showed that the median score of intestinal injury was significantly lower in butyric acid group [1.33(1.33-1.67)] than in PBS group [2.00(1.67-2.25), P<0.05]. qPCR demonstrated that the expressions of IL-6 and TNF-α mRNA were obviously lower in butyric acid group than in PBS group (0.85±0.30 vs. 1.77±0.49 and 0.41±0.25 vs. 0.96±0.56, respectively, P<0.05); and the expressions of IL-10 and TGF-β1 mRNA were markedly higher in butyric acid group than in PBS group (1.91±0.82 vs. 0.94±0.43 and 1.46±0.57 vs. 0.88±0.29, respectively, P<0.05); Intestinal tissue ELISA results showed that the expressions of IL-10 and TGF-β1 were higher in butyric acid group than in PBS group (68.60±15.06 vs. [...]
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