Decrease in IGF-I Binding Sites on Human Promyelocytic Leukemia Cell Line (HL-60) with Differentiation

IZUMI SUKEGAWA, NAOMI HIZUKA, KAZUE TAKANO, KUMIKO ASAKAWA, KAZUO SHIZUME
1987 Endocrinologia Japonica  
Specific insulin-like growth factor I (IGF-I) receptors on human promyelocytic leukemia cell line (HL-60) were identified and characterized. [125I]IGF-I specifically bound to the cells, and PIJIGF-I binding to the cells was displaced by unlabeled IGF-I in a dose dependent manner. [125I] IGF-I binding to the cells were displaced by multiplication stimulating activity (MSA) and porcine insulin, with potencies that were10and100times less than that of IGF-I, respectively. By an affinity labeling
more » ... ffinity labeling technique, IGF type I receptors were found to be present on the HL-60cells. After the cells were differentiated to the macrophage-like cells by 12-o-tetra-decanoyl-phorbol-13-acetate (TPA) and1, 25-dihydroxy-vitamin D3 (1, 25(OH)2D3),[125I]IGF-I binding to the cells decreased significantly. By Scatchard analysis, it was found to be due to a decrease in the number of IGF-I receptors. Thus, the differentiation of HL-60cells to the macrophage-like cells was accompanied by a decrease in IGF-I receptors.
doi:10.1507/endocrj1954.34.365 pmid:2958273 fatcat:km3vtncakzfzpgde36du44s26u