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Identification of specific fragments containing the 5′ end of poliovirus RNA after ribonuclease III digestion
1978
Nucleic Acids Research
The small protein (VPg) covalently linked to the 5' end of poliovirus Type 1 (PV-1) RNA has been labeled in vitro with 125I using the Bolton and Hunter reagent. The RNA is not degraded under the conditions used and nearly all the label enters VPg and not the polynucleotide chain. When this 125I-labeled RNA is cleaved with RNase III at low monovalent salt concentrations, one major 125I-labeled fragment, approximately 100 nucleotides long, is produced. The corresponding fragment from similar
doi:10.1093/nar/5.11.4039
pmid:214765
pmcid:PMC342732
fatcat:hkxieorqwnhfjf7knmz3fab6v4