Inhibition of platelet-tumour cell interaction with ibrutinib reduces proliferation, migration and invasion of lung cancer cells

Zhiqiang Fu, Haifeng Wang, Yi Zhou, Qi Zhou
2018 Tropical Journal of Pharmaceutical Research  
Purpose: To investigate the pharmacological role of the Bruton tyrosine kinase (BTK) inhibitor, ibrutinib, in tumour cell-platelet crosstalk in lung cancer. Methods: Human lung cancer cells A549 were treated with ibrutinib or DMSO. mRNA expression was assessed using reverse transcription-quantitative polymerase chain reaction (RT-PCR), and while western blotting was used to determine protein expression levels. Small interfering RNA (siRNA) transfection was performed to suppress the expression
more » ... ss the expression of galectin-3. Colony formation and Transwell® assays were used to determine cell viability, cell invasiveness and migratory ability. Results: Co-culture of A549 cells and platelets induced activation of BTK/PLCγ2 signalling and subsequent release of PDGF, VEGF and TGFβ1 from de-granulated platelets. However, knocking down of galectin-3 inhibited A549-induced platelet activation. Conversely, platelet activation upregulated the expression of galectin-3 via the release of PDGF. Moreover, ibrutinib significantly (p < 0.05) inhibited cell viability, migration, and invasion. Conclusion: These results suggest that ibrutinib may be a novel therapeutic treatment for lung cancer. This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest
doi:10.4314/tjpr.v17i4.4 fatcat:6mnsjwiz55cydo6wzwp5wn7n34