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A Mutate-and-Map Strategy for Inferring Base Pairs in Structured Nucleic Acids: Proof of Concept on a DNA/RNA Helix
2010
Biochemistry
We propose a rapid chemical strategy for identifying base pairs in structured nucleic acid systems. The approach goes beyond traditional chemical mapping approaches by monitoring perturbations of each residue's chemical accessibility in response to systematic mutagenesis of residues that are distant in sequence but nearby in three dimensions. As a proof of concept, we present highthroughput dimethyl sulfate accessibility data for a chimeric DNA/RNA system in which every possible sequence
doi:10.1021/bi101123g
pmid:20677780
fatcat:t4w5cbzuwjfttij7ihw2hk2tve