Maritalea myrionectae gen. nov., sp. nov., isolated from a culture of the marine ciliate Myrionecta rubra

C. Y. Hwang, K. D. Cho, W. Yih, B. C. Cho
2009 International Journal of Systematic and Evolutionary Microbiology  
Maritalea myrionectae gen. nov., sp. nov., isolated from a culture of the marine ciliate Myrionecta rubra A Gram-negative, rod-shaped, strictly aerobic bacterium, designated strain CL-SK30 T , was isolated from a culture of the marine ciliate Myrionecta rubra. 16S rRNA gene sequence analysis revealed that strain CL-SK30 T was most closely related to Cucumibacter marinus (92.0 % similarity) and next to the type strains of species of the genus Devosia (89.8-91.3 % similarities) in the family
more » ... microbiaceae. Phylogenetic analyses of the 16S rRNA gene sequences showed that strain CL-SK30 T formed a robust clade together with C. marinus, but the sequence divergence value of 8 % between them indicated that the novel bacterium represented a distinct lineage. Strain CL-SK30 T grew optimally in the presence of 2-5 % sea salts at 30-35 6C and pH 7.2-8.0. The major polar lipids were phosphatidylglycerol, diphosphatidylglycerol, two unidentified glycolipids, an unidentified phospholipid and an unidentified lipid. Ubiquinone 10 was the major quinone. The DNA G+C content was 52.7 mol%. Based on its phenotypic, chemotaxonomic and phylogenetic characteristics, strain CL-SK30 T represents a novel genus and species of the family Hyphomicrobiaceae, for which the name Maritalea myrionectae gen. nov., sp. nov. is proposed. The type strain is CL-SK30 T (5KCCM 90060 T 5DSM 19524 T ). The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain CL-SK30 T is EF988631. A transmission electron micrograph of a negatively stained cell of strain CL-SK30 T and two-dimensional thin-layer chromatograms of the polar lipids of strain CL-SK30 T and Cucumibacter marinus CL-GR60 T are available as supplementary material with the online version of this paper.
doi:10.1099/ijs.0.002881-0 pmid:19244448 fatcat:todynapddjhwflgascrp5qz6k4