誗 AIM: To study the relationship between JAK-STAT pathway and epithelial-mesenchymal transition in human lens epithelial cells. Meanwhile, the function of AG490 as a JAK inhibitor was also demonstrated in this article. 誗METHODS: Human lens epithelial cells SRA01 / 04 (LECs) were treated by low concentration of glucose (5. 5mmol / L). High concentration of glucose (30. 5mmol / L) was used to treat the cells in order to form the high glucose model. According to adding AG490 or not, cells were

more »

... ded into the control group and the experimental group, appropriate concentration 10滋 mol / L and 50滋 mol / L of AG490 were chosen and acting time of 6, 12, 24, 48h were selected. Effect of AG490 on cell migration was measured by wound healing test. The expression of TGF-茁 1 , FN, 琢-SMA mRNA were examined by RT-PCR.