Acute exercise in mice transiently remodels the hepatic lipidome in an intensity-dependent manner [post]

Gregory C Henderson, Valeria Martinez Tenorio, Marc A Tuazon
2020 unpublished
Background: The content of triacylglycerol (TAG) in the liver is known to rapidly increase after a single bout of exercise followed by recovery to sedentary levels. The response of other hepatic lipids, and acyl chain composition of lipid classes, would provide a deeper understanding of the response of hepatic lipid metabolism to acute exercise. Methods: Female mice performed a single bout of continuous exercise (CE), high-intensity interval exercise (HIIE), or no exercise (CON). The total
more » ... ON). The total content of various lipids in the liver, and fatty acids within lipid classes, were measured in tissues collected 3 h after exercise (Day 1) and the day following exercise (Day 2). Results: The total concentration of TAG rose on Day 1 after exercise ( P < 0.05), with a greater elevation in HIIE than CE ( P < 0.05), followed by a decline toward CON levels on Day 2. The total concentration of other measured lipid classes was not significantly altered by exercise. However, n-6 polyunsaturated fatty acid relative abundance in diacylglycerol (DAG) was increased by HIIE ( P < 0.05). In CON liver, TAG content was positively correlated with DAG and phosphatidylethanolamine ( P < 0.05), while these statistical associations were disrupted in exercised mice on Day 1. Conclusions: The response of lipid metabolism to exercise involves the coordination of metabolism between various tissues, and the lipid metabolism response to acute exercise places a metabolic burden upon the liver. The present findings describe how the liver copes with this metabolic challenge. The flexibility of the TAG pool size in the liver, and other remodeling of the hepatic lipidome, may be fundamental components of the physiological response to intense exercise.
doi:10.21203/rs.3.rs-62190/v2 fatcat:tv4qizh7arfardrb2u7hrwuree