Long chain triglycerides (LCT) from soya oil have been reported to cause hepatic abnormalities among patients receiving parenteral nutrition. A new source for LCT is needed to support current therapy. The aims of this study were to develop a good and stable superolein oil based intravenous lipid emulsion 20% (SoLE), and to determine its effect on liver status of an animal model. The emulsion was prepared using superolein oil and medium chain triglyceride oil (1:1), 1.2 g lecithin and

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... by high pressure homogenization method. The physical stability study was performed based on realtime, accelerated, force degradation and environmental degradation. Chemical stability was measured based on fatty acids and vitamin E contents, peroxide value and acidity value. As the animal model, three rabbits were administered with 1.5 g/kg SoLE for 5 h and repeated daily for three days. The SoLE was found to have good physicochemical characters: 229.07±1.57 nm of particle size; 0.00% of particle > 5 µm; 342.00±1.00 mOsm/kg of osmolarity; -46.67±4.40 mV of zeta potential; 1.72±0.04 cP of viscosity; 7.83±0.06 of pH; 0.80±0.06 kcal/g calorie; 1.74±0.05 mEqO 2 /kg of peroxide value; and 0.03±0.01% of acidity value, and excellent physicochemical stabilities for up to six months of storage at various temperatures and conditions. The level of plasma lipid and plasma liver enzymes profiles of the rabbits remained statistically unchanged. This study has successfully developed a stable SoLE 20% and it was found to be safe to the liver of the animal model. ABSTRAK Trigliserida rantaian panjang (LCT) daripada minyak kacang soya telah dilapor menyebabkan keabnormalan hepatik dalam kalangan pesakit yang menerima nutrisi parenteral. Sumber baharu untuk LCT diperlukan bagi menyokong terapi semasa. Tujuan kajian ini adalah untuk membangunkan emulsi lipid intravena 20% (SoLE) berasaskan minyak superolein yang baik dan stabil dan menentukan kesannya terhadap status hati model haiwan. Emulsi telah disediakan dengan menggunakan minyak superolein dan minyak trigliserida rantaian sederhana (1: 1), 1.2 g lesitin dan dihomogenkan dengan kaedah penghomogenan tekanan tinggi. Kajian kestabilan fizikal dijalankan berdasarkan masa sebenar, dipercepat, degradasi paksa dan degradasi persekitaran. Kestabilan kimia dihitung berdasarkan kandungan asid lemak, kandungan vitamin E, nilai peroksida dan nilai keasidan. Sebagai model haiwan, tiga ekor arnab diberikan 1.5 g/kg SoLE selama 5 jam dan diulang setiap hari selama tiga hari. SoLE didapati mempunyai sifat fizikokimia yang baik iaitu 229.07±1.57 nm saiz zarah, 0.00% zarah bersaiz melebihi 5 µm, 342.00±1.00 mOsm/kg osmolariti, -46.67±4.40 mV potensi zeta, 1.7 ±0.04 cP kelikatan, 7.83±0.06 pH, 0.80±0.06 kcal/g kalori, 1.74±0.05 mEqO 2 /kg nilai peroksida dan 0.03±0.01% nilai keasidan serta kestabilan fizikokimia pula adalah cemerlang sehingga enam bulan penyimpanan pada pelbagai suhu dan keadaan. Tahap profil lipid plasma dan enzim hati plasma haiwan yang dikaji kekal tidak berubah secara statistik. Kajian ini telah berjaya membangunkan SoLE 20% yang stabil dan selamat kepada hati model haiwan. Kata kunci: Emulsi lipid intravena; kestabilan fizikokimia; minyak superolein; model haiwan