Screening and Fermentation of Jinggang-mycin High-yielding Strains

Xiaoju Zhang, Shijie Li, Jiangrong Xiao
2018 Proceedings of the 2018 7th International Conference on Energy and Environmental Protection (ICEEP 2018)   unpublished
Using KN-16 as original strains, adopting compound mutagenic of ultraviolet irradiation, 60 Coγ-ray irradiation, protoplast ultraviolet irradiation, measuring the content of Jinggang-mycin A by gas chromatography. The mutagenesis condition was determined by lethality testing, flask fermentation, resistance screening of Jinggang-mycin A. When ultraviolet irradiation time at 9s, 60 Coγ-ray dose at 300 Gy, we got strains of K-108, prepared the protoplast, ultraviolet irradiation time at 15s, and
more » ... reening of tolerated maximum Jinggang-mycin A concentration of 5.5g/100mL, We got stable high-yield strains of 2-27-1, its Jinggang-mycin A concentration in biomass was 4.48g/100mL. Compared with the original strains, the production increased 134.6%. For the mutant strains in this study, the colony morphology is irregular, yellow, light-colored, and no obvious intermediate water absorbent, the growth of colonies in the resistance plate is small, this is different to traditional view.
doi:10.2991/iceep-18.2018.90 fatcat:zphfkvkel5f5xeucsld7oonbyq