Influence of ultrasonic intensity on sinking of Microcystis colonies and their floating process under different light and temperature conditions Laboratory of Integrated Regulation and Resource Development on Shallow Lake of Ministry of Education, College of Environment, Hohai University, Nanjing 210098, P.R.China) ( 2: State Key Laboratory of Lake Science and Environment, Nanjing Institute of Geography and Limnology, Chinese Academy of Sciences, Nanjing 210008, P.R.China) Abstract: Inhibition

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... ffect of different ultrasonic intensities was investigated by using Microcystis colonies from Lake Taihu, and the aim of this study was to obtain the optimal ultrasound parameters. The floating character of Microcystis colonies after sonication was cultured under different illumination intensity and temperature conditions. Results showed that sonication for 60 s at 0.0353 W / cm 3 was an optimal parameter, which led to an immediate sedimentation ( up to 80% ) markedly. In the experiment, the algal cell con• centration and chlorophyll•a concentration were unchanged, which suggested that algal cells remained intact and cell inclusions did not leak to contaminate water quality. Light and temperature played important roles in the floating process of Microcystis colonies, but light was a more significant factor during the process. Under 2000 lx at 25℃ ,the floating rate of algal cells could restore to the normal level after 120 h, while the floating rate of algal cells after sonication did not change significantly at a relatively low temper• ature ( ≤20℃ ) . In comparison, at a higher temperature of 25℃ , the floating rate increased after 72 h which could restore to 80% of the level in control group after 120 h. Therefore, the ultrasonic control of algal blooms in lakes should choose a weak illumination intensity and low temperature condition. Keywords: Microcystis colonies; ultrasonic intensity; exposure time; sedimentation; floating rate; illumination; temperature; Lake Taihu * 国家自然科学基金项 目 ( 31470507 ) 、 中 央 高 校 科 研 业 务 费 项 目 ( 2013B32414 ) 和 江 苏 省 优 势 学 科 平 台 项 目 ( PAPD) 联合资助. 2016 07 25 收稿; 2016 12 02 收修改稿. 谭啸(1980 ~ ),男,博士,副教授; E•mail: biotan@ 163.com.