Phage displayed domain antibody mimics for pyrethroid and its application in immunoassay

Yanyan Zhao, 1. School of Food Science and technology, Henan Institute of Science and Technology, Xinxiang 453003, China, Ying Liang, Yuan Liu, Sicong Tu, Shengming Zhao, Xianjin Liu, Kang Tu, 2. Key Laboratory of Food Quality and Safety of Jiangsu Province-State Key Laboratory Breeding Base, Key Laboratory of Control Technology and Standard for Agri-product Safety and Quality, Ministry of Agriculture and Rural Affairs, Institute of Agricultural Product Quality Safety and Nutrition Research, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China, 3. College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China, 4. School of Medical Sciences, University of New South Wales, Sydney, NSW 2052, Australia
2020 International Journal of Agricultural and Biological Engineering  
Anti-idiotypic antibodies which can mimic antigens have many potential applications in the immunoassay. This research used the monoclonal antibody of the conserved chemical region of pyrethroids, 3-phenoxybenzoic acid (PBA), and the domain antibody library to develop an environmentally-friendly immunoassay for the detection of pyrethroids. The domain antibodies (A8, B8, and C6) which bound to anti-PBA monoclonal antibody (MAb) were isolated from a naive phage display human domain antibody
more » ... y. This domain antibody is cloneable, pyrethroid-free, and applicable as a competitive mimetic antigen in the immunoassay. The best immunoassay was achieved using A8, resulting in IC 50 of 0.714 μg/mL for PBA, 1.775 μg/mL for Cypermethrin, 1.624 μg/mL for β-cypermethrin, 3.675 μg/mL for Fenvalerate, and 4.895 μg/mL for Flucythrinate. This way of selecting anti-idiotypic antibodies to detect pyrethroids could provide potential applications in developing immunoassays for identifying various chemical contaminants in food. et al. Phage displayed domain antibody mimics for pyrethroid and its application in immunoassay. Int J Agric & Biol Eng, 2020; 13(2): 235-240.
doi:10.25165/j.ijabe.20201302.5492 fatcat:toqox6ltm5fs3avkc4ikrumpgq