In this paper , we propose a semi − closed microchipwhich can r 巳 al 〒 ze the probe type manipula 匸ion in the microchip . The sem { − c 工 osed mlcl ' ochip has a bath in the middle of rnicrochannel to allow the insert. ion Df rnicropipettes 「 nto the microcl 】ip . The bath is sealed off by thin oil film to preven しthe cvaporation of thc selu 仁 ien in the bath、 The scal by thc oil film can main ヒ ai 正 1 evcn if microp 重 pettcs are inserted int ⊂ ) the bath and pulled out from the bath. The seal

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... y the oil film was 巳valuated by meas ' uring thc weight ef the bath after 12 〆 24 hQur5. To LLse the scmi − closed microchip for cell ct lturc and cell analysis , the exchunge of 日 1e solution in the semi − cbsed micr ') chip was demonstrated. The cell 丘xation with the thermo sensitive gel was also demonstrated hy fabricating the ITQ electrodes as heaters iTl the bath. Cell cu 比ure and the target ccll harvesting were cund ロ ete [ 1 by the semi − closed micT 〔 〕 chip . The proposed semi − cl ' 〕sed microchip ca1 、 be used for cell culture , cen analysis and cel ] haTvest 量 ng under a microscope as