Steady-state mRNA levels of the sarcolemmal Na(+)-Ca2+ exchanger peak near birth in developing rabbit and rat hearts

S R Boerth, D B Zimmer, M Artman
1994 Circulation Research  
were normalized by reprobing the same blots with 32P 5'-endlabeled oligo(dT). In both species, exchanger mRNA levels peaked near birth and declined postnatally. Maximal levels were approximately sixfold greater in the late fetal rabbit (gestational day 29) and eightfold greater in the early newborn rat (postnatal day 1) compared with adults of the respective species. The parallel changes in exchanger mRNA and protein levels suggest that developmental regulation of cardiac Na+-Ca 2+ exchanger
more » ... ression involves pretranslational control mechanisms. These results support the concept that during normal cardiac development, Na+-Ca 2+ exchanger expression is maximal near the time of birth and then declines postnatally as Ca 2+ regulation by the sarcoplasmic reticulum reaches functional maturity. (Circ Res. 1994;74:354-359.) Key Words * fetus * newborn * cardiac development -Na+-Ca2+ exchanger developing rabbit and rat hearts. Steady-state mRNA levels of the sarcolemmal Na(+)-Ca2+ exchanger peak near birth in
doi:10.1161/01.res.74.2.354 pmid:8293573 fatcat:efixddkocbhzrfcls6vy2bcnui