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Changes in the concentration of free calcium ions, in the cytoplasm, [Ca2+]j, are used to trigger or regulate a wide variety of cellular functions. Methods for measuring the [Ca2+}i are thus important tools for investigating cell activation. Two factors make these measurements difficult. Compared with the free concentrations of the potentially interfering cations, LFF, Mg2, Na and K, [Ca2]i is very low being around 10' MM at rest and rising perhaps to lO M on maximum activation. The detectiondoi:10.1351/pac198355121977 fatcat:ifv2giaykvduzkbtfvfv7ojdom