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Direct and indirect selection pressures imposed by antibiotics and co-selective agents and horizontal gene transfer are fundamental drivers of the evolution and spread of antibiotic resistance. Therefore, effective environmental monitoring tools should ideally capture not only antibiotic resistance genes (ARGs), but also mobile genetic elements (MGEs) and indicators of co-selective forces, such as metal resistance genes (MRGs). A major challenge towards characterizing the potential human healthdoi:10.1186/s40168-019-0703-9 pmid:31174603 pmcid:PMC6555988 fatcat:jfvsbc6emfdhjd56lyuvvm44yu