Photosynthetic and biochemical characterization of in vitro-derived African violet (Saintpaulia ionanthaH. Wendl) plants to ex vitro conditions

Yaser Hassan Dewir, Mohammed El-Sayed El-Mahrouk, Hanady Salim Al-Shmgani, Hail Z. Rihan, Jaime A. Teixeira da Silva, Michael P. Fuller
2015 Journal of Plant Interactions  
African violet (Saintpaulia ionantha H. Wendl) is one of the most easily and commonly tissue-cultured ornamental plants. Despite this, there are limited reports on photosynthetic capacity and its impact on the plant quality during acclimatization. Various growth, photosynthetic and biochemical parameters and activities of antioxidant enzymes and dehydrins of micropropagated plants were assessed under three light intensities (35, 70, and 100 µmol m −2 s −1 photosynthetic photon flux density
more » ... n flux density -PPFD). Fresh and dry plant biomass, plant height, and leaf area were optimal with high irradiance (70-100 µmol m −2 s −1 PPFD). Chlorophyll and carotenoid contents and net photosynthesis were optimal in plants grown under 70 µmol m −2 s −1 PPFD. Stomatal resistance, malondialdehyde content, and F v /F m values were highest at low light irradiance (35 µmol m −2 s −1 PPFD). The activities of three antioxidant enzymes, superoxide dismutase, catalase, and glutathione peroxidase, increased as light irradiance increased, signaling that high light irradiance was an abiotic stress. The accumulation of 55, 33, and 25 kDa dehydrins was observed with all light treatments although the expression levels were highest at 35 µmol m −2 s −1 PPFD. Irradiance at 70 µmol m −2 s −1 PPFD was suitable for the acclimatization of African violet plants. Both low and high irradiance levels (35 and 100 µmol m −2 s −1 PPFD) induced the accumulation of antioxidants and dehydrins in plants which reveals enhanced stress levels and measures to counter it.
doi:10.1080/17429145.2015.1018967 fatcat:uvjwok5wvrfa7ncz7iojsz5vui