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Agrobacterium Tumefaciens Mediated Transformation of rolC::Hd3a-GFP in Black Rice (Oryza Sativa L. cv. Cempo Ireng) to Promote Early Flowering
2015
Procedia Chemistry
The purpose of this study was to transform rolC::Hd3a-GFP by Agrobacterium tumefaciens on black rice (Oryza sativa L. cv. Cempo Ireng). The callus formation were induced from scutellum using two different media, 2N 6 and 2,4-dichlorophenoxyacetic acid (2,4-D). PCR analysis using specific primers for Hd3a and hpt was performed to determine the stability of rolC::Hd3a-GFP construct before co-cultivation of callus and Agrobacterium tumefaciens. This study indicated that black rice callus induction
doi:10.1016/j.proche.2015.03.063
fatcat:v7n5qazlo5bu7hd3timcswphuu