Agrobacterium Tumefaciens Mediated Transformation of rolC::Hd3a-GFP in Black Rice (Oryza Sativa L. cv. Cempo Ireng) to Promote Early Flowering

Yekti Asih Purwestri, Resta Dewi Komala Sari, Lisa Novita Anggraeni, Aries Bagus Sasongko
2015 Procedia Chemistry  
The purpose of this study was to transform rolC::Hd3a-GFP by Agrobacterium tumefaciens on black rice (Oryza sativa L. cv. Cempo Ireng). The callus formation were induced from scutellum using two different media, 2N 6 and 2,4-dichlorophenoxyacetic acid (2,4-D). PCR analysis using specific primers for Hd3a and hpt was performed to determine the stability of rolC::Hd3a-GFP construct before co-cultivation of callus and Agrobacterium tumefaciens. This study indicated that black rice callus induction
more » ... on 2N 6 medium responded faster than that on MS 2,4-D medium and generated friable calli for Agrobaterium-mediated transformation. The screening result showed that several calli were competent to regenerate on medium containing hygromysin and demonstrated Hd3a insertion gene.
doi:10.1016/j.proche.2015.03.063 fatcat:v7n5qazlo5bu7hd3timcswphuu