Effects and mechanism of high ARNO expression on the permeability of human renal glomerular endothelial cells under high glucose condition

Xiao-Long WANG, Cheng-Fang HU, Lu-Yao TANG, Yi LI, Tao GUAN, Yun-Jian HUANG
2020 Medical Journal of Chinese People's Liberation Army  
Objective To explore the effects and mechanism of high expression of ARNO (ARF nucleotide-binding-site opener) on the permeability of human renal glomerular endothelial cells (HRGECs) under high glucose conditions. Methods HRGECs were cultured in vitro, and ARNO expression and endothelial permeability were detected in high glucose time gradient (15, 30, 45 and 60 min) and concentration gradient (10, 20, 30 and 40 mmol/L) experiments, respectively. Cell lines of HRGECs with ARNO and Arf6 gene
more » ... NO and Arf6 gene silencing were obtained by infecting HRGECs with ARNO siRNA and Arf6 siRNA recombinant lentivirus vectors, respectively. The effects of ARNO and Arf6 gene silencing on endothelial permeability were observed. Results In the time gradient experiments, compared with control group, ARNO expressions increased significantly in the groups with high glucose for 15, 30, 45 and 60 min (0.670±0.051, 0.960±0.106, 0.716±0.026, 0.531±0.030 vs. 0.242±0.029. P<0.05). Compared with high glucose 15 min group, ARNO expression increased in high glucose 30 min group (P<0.05); Compared with high glucose 30 min group, ARNO expression decreased in high glucose 45 min group (P<0.05); Compared with high glucose 45 min group, ARNO expression decreased in high glucose 60 min group (P<0.05). Compared with control group, endothelial permeability increased significantly in the groups with high glucose for 15, 30, 45 and 60 min (1.196±0.004, 1.399±0.012, 1.301±0.052, 1.184±0.030 vs. 1.000, P<0.05). Compared with high glucose 15 min group, endothelial permeability increased in high glucose 30 min group (P<0.05); Compared with high glucose 30 min group, endothelial permeability decreased in high glucose 45 min group (P<0.05); Compared with high glucose 45 min group, endothelial permeability decreased in high glucose 60 min group (P<0.05). In the concentration gradient experiments, compared with normal concentration glucose group, ARNO expressions increased significantly in the groups with high glucose of 20, 30 and 40 mmol/L (0.632±0.031, 0.927±0.041, 1.183± [...]
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