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Specific Substitutions at Amino Acid 256 of the Sarcoplasmic/Endoplasmic Reticulum Ca2+Transport ATPase Mediate Resistance to Thapsigargin in Thapsigargin-resistant Hamster Cells
Journal of Biological Chemistry
High levels of resistance to thapsigargin (TG), a specific inhibitor of intracellular Ca 2؉ transport ATPases (SERCAs), can be developed in culture by stepwise exposure of mammalian cells to increasing concentrations of TG. We have identified, in two independently selected TG-resistant hamster cell lines of different lineages, mutant forms of SERCA. In the TG-resistant Chinese hamster lung fibroblast cell line DC-3F/TG, a T 3 C change at nucleotide 766 introduces a Phe 256 3 Leu alterationdoi:10.1074/jbc.273.6.3542 pmid:9452480 fatcat:sekcwt5mrveftfzs5mxbwspldy