APPARENT ENDOXYLANASE ACTIVITY IN RYE CULTIVARS FROM LITHUANIA

Daiva Vidmantiene, Grazina Juodeikiene, Hilmer Sørensen, Charlotte Bjergegaard
2008 unpublished
The efficiency of cereal-based biotechnological processes closely linked to both their chemical composition and selection of cell-wall degrading enzymes. Degradation of cell-wall polysaccharides during processing, by means of added or naturally occurring endogenous xylanases, may also affect the quality of the end-product. The aim of the present study was determination of the apparent activity of endogenous xylanases in four winter rye varieties and in two newly developed rye hybrids. The
more » ... hybrids. The influence of variety and climatic conditions on apparent endoxylanase activity was investigated. Rye cultivars were grown on different soils at three Plant Breeding Stations of Lithuania in 2003 and 2004. The apparent endoxylanase activity was studied in the albumin type extracts from meal of the rye kernels (pH 4.5; 40 °C) by an assay based on dinitrosalicylic acid. The arabinoxylan used as substrate was isolated from wheat bran, and the reducing sugars were purified by cation-exchange chromatography. The results showed the significant influence of the genotype and agro-climatic conditions on the endoxylanase activity varying between 5.1±0.6 and 23.6±0.9 nkat/g grain. No significant correlations were found between Falling number (FN) and apparent endoxylanase activity in rye.
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