This SOP outlines guidelines on how to process the isolates for the 2022 GenomeTrakr (GT) Proficiency Testing exercise. This SOP is applicable to all GenomeTrakr labs participating in the 2022 GenomeTrakr Proficiency Testing exercise (PulseNet Harmonized). The FDA GenomeTrakr program will ship the following proficiency testing isolates on April, 2022. ESP22-5191 Shigella SAP22-0236 Salmonella SAP22-0692 Salmonella SAP22-1300 Salmonella Completion of the entire proficiency test entails the

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... ing: GT participating laboratory generates sequencing data (fastq files) using the PT strains provided by CDC through FDA-CFSAN-WGS Program-GT in accordance to GT and/or PulseNet SOPs. Populate sample sheet according to 2022 GT Proficiency Testing exercise (PulseNet Harmonized) SOP Submission of sequencing records to the appropriate project on BaseSpace or Isilon according to GT SOPs By participating in the 2022 GT Proficiency Testing exercise (PulseNet Harmonized), GT labs provide consent to use the PT exercise data in subsequent analysis and manuscript publications. Participants will be acknowledged for their contribution on any publication that might require processing data from the 2022 GT PT exercise. v5: Added links to GalaxyTrakr_PT_exercise_report_2022.xlsx