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Quantitation of RNA coliphage inactivated by UV radiation using RT-PCR method
UV照射により不活化されたRNAファージのRT‐PCR法による定量
1997
Environmental Engineering Research
UV照射により不活化されたRNAファージのRT‐PCR法による定量
PCR (polymerase chain reaction) is a useful method for viral monitoring because of its high sensitivity and short time detection. However, disadvantages of PCR method for viral monitoring are pointed out to be lack of quantification and no differentiation of infectious viruses from noninfectious viruses. MPN method using RT(reverse transcription)-PCR was applied for enumeration of RNA coliphage QƒÀ, and nested PCR was applied to promote the sensitivity of RT-PCR. This method gave consistently
doi:10.11532/proes1992.34.83
fatcat:zl2jxzbujzf6tbtfwdspnytzam