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... gers, des laboratoires publics ou privés. Microscopic investigation of changes in histology and digestibility in the rumen of a forage grass and a forage legume during the first growth stage J Jamot, E Grenet To cite this version: J Jamot, E Grenet. Microscopic investigation of changes in histology and digestibility in the rumen of a forage grass and a forage legume during the first growth stage. Summary ― An Italian &dquoDalita&dquo ryegrass (Lolium italicum) and a European lucerne (Medicago sativa) were harvested at 5 different growth stages to determine the anatomical factors limiting their digestibility and in particular the effects of lignification of the tissues. In vitro digestibility, cell wall contents of the whole plant and stem of lucerne and of the whole plant, stem and leaf blade of ryegrass were determined. The rate and the extent of degradation in the rumen of the different tissues were observed by scanning electron microscopy. This degradation occurred very rapidly with the lucerne stems; the xylem of lucerne was the only undegradable tissue whatever the stage. The collenchyma was degraded in the rumen although with acid phloroglucinol it stained positive for the presence of phenolic compounds. Ryegrass stems were digested more slowly than lucerne stems, and the sclerenchyma and xylem of ryegrass were indigestible whatever the stage. The parenchyma located close to the sclerenchyma became indigestible as the cell walls lignified progressively from the third stage. These results contribute to the understanding of the decrease in digestibility over the first growth stage and the variation in rate of digestion of lucerne and ryegrass in the rumen. * Correspondence and reprints gressivement lignifiées. Ces résultats contribuent à expliquer la diminution de la digestibilité observée avec l'âge de la plante, et les différences de vitesse de dégradation dans le rumen de la luzerne et du ray-grass. graminée / légumineuse / digestiblité / lignification / microscopie