Antiviral proteins were isolated, from host plants, following treatment with an antiviral glycoprotein, extracted from the roots of Boerhaavia diffusa plants. It was found to be active against isometric as well as an isometric viruses in their respective hypersensitive and systemic hosts. The biologically active principal was isolated and purified. Estimation of proteins, from the leaf samples of N.glutinosa plants, indicated higher concentration of new proteins in plants whose two basal leaves

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... were treated 24 hours earlier with an aqueous solution of the glycoprotein. SDS-PAGE analysis studies on partially purified antiviral principal of protein nature revealed that the size of band in L 3 (after 24 hours) was 30 Kda along with very dark and prominent bands. The size of band recorded in rest treatments was of 36 Kda and 50 Kda. About 200 gram fresh, non treated upper leaves of N. glutinosa plants, two basal leaves on the same plant had been sprayed 24