Objective:JuglansregiaL. (Common Walnut), Juglandaceae, is one of important medicinal plants. It is used to treat skin inflammations, ulcers and venous insufficiency. Its bark is used by Iraqi women to obtain a natural red-brown lip color, but there are no previous studies to use its natural pigment as a histological dye. This study aims to find new natural dye to be as alternative for synthetic dye. Materials and Methods:Paraffin sections of rat kidney, liver and spleen were prepared according

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... the routine histological work and used in this study.Ethanolic extractof J. regia bark was prepared and used as a counterstain after Harris haematoxylin. Results:After microscopic examination,J. regiaextract showed strong affinity for the cytoplasm. Cell nuclei were stained by haematoxylin while the cytoplasm was stained by J. regia extract. This affinitycan be inferred that the plant extract has an acidic nature due to its polyphenoliccompounds. Conclusion: J. regia extract is a good natural alternative for eosin