Regulation of branched-chain amino acid transport in Escherichia coli

S C Quay, D L Oxender
1976 Journal of Bacteriology  
The repression and derepression of leucine, isoleucine, and valine transport in Escherichia coli K-12 was examined by using strains auxotrophic for leucine, isoleucine, valine, and methionine. In experiments designed to limit each of these amino acids separately, we demonstrate that leucine limitation alone derepressed the leucine-binding protein, the high-affinity branched-chain amino acid transport system (LIV-I), and the membrane-bound, low-affinity system (LIV-II). This regulation did not
more » ... em to involve inactivation of transport components, but represented an increase in the differential rate of synthesis of transport components relative to total cellular proteins. The apparent regulation of transport by isoleucine, valine, and methionine reported elsewhere was shown to require an intact leucine biosynthetic operon and to result from changes in the level of leucine biosynthetic enzymes. A functional leucyltransfer ribonucleic acid synthetase was also required for repression of transport. Transport regulation was shown to be essentially independent of ilvA or its gene product, threonine deaminase. The central role of leucine or its derivatives in cellular metabolism in general is discussed.
doi:10.1128/jb.127.3.1225-1238.1976 fatcat:a6bobjj5n5f47kxch7grotdstu