Determinação de bussulfano em plasma através de cromatografia líquida de alta eficiência com detector de arranjo de diodos e derivatização com dietilditiocarbamato de sódio

Charline Fernanda Backes, Sandrine Comparsi Wagner, Fernando Dal Pont Morisso, Marina Venzon Antunes, Rafael Linden, Marcelo Capra
2012 Química Nova  
Recebido em 5/1/12; aceito em 13/2/12; publicado na web em 30/4/12 DETERMINATION OF BUSULFAN IN PLASMA BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY WITH DIODE ARRAY DETECTOR AND DERIVATIZATION WITH SODIUM DIETHYLDITHIOCARBAMATE. A high performance liquid chromatographic-diode array detection method for the determination of busulfan in plasma was developed and validated. Sample preparation consisted of protein precipitation followed by derivatization with sodium diethyldithiocarbamate and
more » ... uid extraction with methyl-tert-butyl ether. Chromatograms were monitored at 277 nm. Separation was carried out on a Lichrospher RP 18 column (5 µm, 250 x 4 mm). The mobile phase consisted of water and acetonitrile (20:80, v/v). The method presented adequate specificity, linearity, precision and accuracy and allowed reliable determination of busulfan in clinical plasma samples, being applied to three patients submitted to bone marrow transplantation.
doi:10.1590/s0100-40422012000700031 fatcat:icrcawcidnh5bojm662bcexbmy