Dynamic clamp with StdpC software

Ildikó Kemenes, Vincenzo Marra, Michael Crossley, Dávid Samu, Kevin Staras, György Kemenes, Thomas Nowotny
2011 Nature Protocols  
Dynamic clamp is a powerful method that allows the introduction of artificial electrical components into target cells to simulate ionic conductances and synaptic inputs. This method is based on a fast cycle of measuring the membrane potential of a cell, calculating the current of a desired simulated component using an appropriate model and injecting this current into the cell. Here, we present a dynamic clamp protocol using free, fully integrated, open-source software (StdpC, Spike timing
more » ... ent plasticity Clamp). Use of this protocol does not require specialist hardware, costly commercial software, experience in real time operating systems or a strong programming background. The software enables the configuration and operation of a wide range of complex and fully automated dynamic clamp experiments via an intuitive and powerful interface with a minimal initial lead-time of a few hours. After initial configuration, experimental results can be generated within minutes of cell impalement.
doi:10.1038/nprot.2010.200 pmid:21372819 pmcid:PMC3188375 fatcat:fzyhq3hpvzbprjxwyhw6qn6llq