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Consortium of bacteria capable of degrading oily hydrocarbons were isolated from tarball on beaches in Terengganu, Malaysia and classified as Pseudomonas stutzeri, Cellulosimicrobium cellulans, Acinetobacter baumannii and Pseudomonas balearica. Taguchi design was used to optimize diesel-oil biodegradation using these bacteria as consortium. Maximum diesel-oil biodegradation by experimental runs was 93.6% with individual n-alkanes degraded between 87.6% – 97.6% in 30 days. Optimal settings weredoi:10.21203/rs.3.rs-107265/v1 fatcat:oz74zfmrmva3vmw65vq2kbc2pe