We present a platform for micro particle image velocimetry (mPIV), capable of carrying out fullchannel, temperature-controlled, long-term-stable, and automated mPIV-measurement of microchannel acoustophoresis with uncertainties below 5% and a spatial resolution in the order of 20 mm. A method to determine optimal mPIV-settings for obtaining high-quality results of the spatially inhomogeneous acoustophoretic velocity fields of large dynamical range is presented. In particular we study the

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... nce of the results on the mPIV interrogation window size and the number of repeated experiments. The mPIV-method was further verified by comparing it with our previously published particle tracking method. Using the mPIV platform we present a series of high-resolution measurements of the acoustophoretic velocity field as a function of the driving frequency, the driving voltage, and the resonator temperature. Finally, we establish a direct and consistent connection between the obtained acoustophoretic velocity fields, and continuous flow mode acoustophoresis, commonly used in applications.