Comparative interactomics analysis of protein family interaction networks using PSIMAP (protein structural interactome map)

D. Park, S. Lee, D. Bolser, M. Schroeder, M. Lappe, D. Oh, J. Bhak
2005 Bioinformatics  
Motivation: Many genomes have been completely sequenced. However, detecting and analyzing their proteinprotein interactions by experimental methods such as coimmunoprecipitation, tandem affinity purification, and Y2H is not as fast as genome sequencing. Therefore, a computational prediction method based on the known protein structural interactions will be useful to analyze large-scale protein-protein interaction rules within and among complete genomes. Results: We confirmed that all the
more » ... hat all the predicted protein family interactomes (the full set of protein family interactions within a proteome) of 146 species are scale-free networks, and they share a small core network comprising 36 protein families related to indispensable cellular functions. We found two fundamental differences among prokaryotic and eukaryotic interactomes: 1) eukarya had significantly more hub families than archaea and bacteria, and 2) certain special hub families determined the topology of the eukaryotic interactomes. Our comparative analysis suggests that a very small number of expansive protein families led the evolution of interactomes and seemed to have played a key role in species diversification.
doi:10.1093/bioinformatics/bti512 pmid:15914543 fatcat:nrsvweppdvgxjidkixxitemgeq