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Cloning and sequencing of the xylose isomerase and xylulose kinase genes of Escherichia coli
1984
Applied and Environmental Microbiology
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A 4.2-kilobase-pair fragment of the Escherichia coli chromosome which contains the genes for xylose isomerase and xylulose kinase was cloned into plasmid pBR322. The hybrid plasmid (designated pECX14) complements strains deficient in either or both of the two enzymes. Deletion derivatives of pECX14 were used to localize the two genes on the cloned fragment. The entire nucleotide sequence of the cloned fragment was determined. Open reading frames which, if translated, would encode proteins of
doi:10.1128/aem.47.1.15-21.1984
fatcat:7etbq7to4bfvhkntpclacg3jni