Hepatitis B is prevalent in khairpure Mirus, Pakistan, yet up-to-date data on the epidemiology of hepatitis B in Khairpure Mir's catchment area of Teaching hospital, KMC, khairpur, data is not available before in this catchment area. Methodology: One thousand, three hundred and ninety seven patients were referred to pathological laboratory of kmc teaching hospital khairpur Pakistan, who were suspected for hepatitis HBV, from different OPDs, and data was collected from January 2017 to august

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... from which Eight hundred ninety eight were positive on Elisa method, were screened for HBV. Patients of either sex, with more than thirty years of age were included. From which 898 HBV were positive on Elisa method. Result: Data was collected from January 2017 to August 2017 from which were Eight hundred ninety eight were positive on Elisa method, were screened for HBV and HCV. Patients of either sex, with more than thirty years of age were included. From which 898 HBV were positive on Elisa method OUT OF thirteen hundred and ninety seven advised for hepatitis B. Means 64% is very high prevalence. Conclusion: Data suggest a moderate to high prevalence of HBV, at KMC Hospital catchment area Khairpure Mirs, Pakistan. The modes of transmission of hepatitis B in Pakistan implicate contaminated needle used in medical care, drug abuse, unsafe blood and blood product transfusion, and blood bank running in the town without qualified pathologists, as the major causing factors.