INVESTIGATION OF THE BIOCIDAL EFFECT OF ELECTROCHEMICALLY ACTIVATED AQUEOUS SODIUM CHLORIDE SOLUTION ON STAPHYLOCOCCUS AUREUS

Teodora Popova, Toshka Petrova, Stoil Karadzhov, Ganeta Krustanova
2016 TRADITION AND MODERNITY IN VETERINARY MEDICINE   unpublished
Studies were carried out to determine the sensitivity of Staphylococcus aureus to electrochemically activated 3% aqueous sodium chloride solution (anolyte) in different concentrations-100 %, 50 %, 25 % and 12.5 %. As a control was used the disinfectant Virkon S, applied at final concentrations of 1 %, 0.5 %, 0.25 % and 0.125 %. Two referent strains of S. aureus were used-ATCC and Kowan. It had been found that the anolyte in concentrations of 50 and 100 % inactivates the cells of S. aureus ATCC
more » ... of S. aureus ATCC in suspension at a density of 106 cells/ml within 5 min. After 10 minutes of impact and lower concentrations (25 and 12.5 %) had a bactericidal effect. The anolyte in all tested concentration (12.5 to 100 %) had a bactericidal effect on the cells of S. aureus Kowan in suspension with concentration of 106 cells/ml in 10-minutes. Shorter intervals tested (2 min and 5min) were not sufficient for achieving bactericidal action even at a concentration of anolyte 50 and 100 %, while after 10 min and even smaller concentrations (25 and 12.5 %) had such action. S. aureus ATCC showed slightly higher sensitivity to anolyte and Virkon S compared to the other tested strain Kowan. The effect of the control disinfectant Virkon S on the tested staphylococcal strains was completely analogous to that of the anolyte.
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